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Computational design of allosteric ribozymes as molecular biosensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
14302 1205 2014 13 PDF Available
Title
Computational design of allosteric ribozymes as molecular biosensors
Abstract

Nucleic acids have proven to be a very suitable medium for engineering various nanostructures and devices. While synthetic DNAs are commonly used for self-assembly of nanostructures and devices in vitro, functional RNAs, such as ribozymes, are employed both in vitro and in vivo. Allosteric ribozymes have applications in molecular computing, biosensoring, high-throughput screening arrays, exogenous control of gene expression, and others. They switch on and off their catalytic function as a result of a conformational change induced by ligand binding. Designer ribozymes are engineered to respond to different effectors by in vitro selection, rational and computational design methods. Here, I present diverse computational methods for designing allosteric ribozymes with various logic functions that sense oligonucleotides or small molecules. These methods yield the desired ribozyme sequences within minutes in contrast to the in vitro selection methods, which require weeks. Methods for synthesis and biochemical testing of ribozymes are also discussed.

Keywords
Hammerhead ribozymes; Allosteric ribozymes; Nucleic acid engineering; Molecular biosensors; Computational design; RNA length-sensing ribozymes; High-speed allosteric ribozymes; Oligonucleotide-sensing ribozymes; Small molecule-sensing ribozymes; RNA-based
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 32, Issue 5, September–October 2014, Pages 1015–1027
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us