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In vitro study of the proliferation and growth of human bone marrow cells on apatite–wollastonite-2M glass ceramics

Paper ID Volume ID Publish Year Pages File Format Full-Text
1431 83 2010 10 PDF Available
Title
In vitro study of the proliferation and growth of human bone marrow cells on apatite–wollastonite-2M glass ceramics
Abstract

This study concerns the preparation and in vitro characterization of an apatite–wollastonite-2M bioactive glass ceramic which is intended to be used for the regeneration of hard tissue (i.e. in dental and craniomaxillofacial surgery). This bioglass ceramic has been obtained by appropriate thermal treatment through the devitrification (crystallization) of a glass with a stoichiometric eutectic composition within the Ca3(PO4)2–CaSiO3 binary system. Crack-free specimens of the bioglass ceramic were immersed in human bone marrow cell cultures for 3, 7, 14 and 21 days, in order to study biocompatibility. Cell morphology, proliferation and colonization were assessed by scanning electron microscopy and confocal laser scanning microscopy. A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells. The results showed that the cells were able to adhere and proliferate on the designed material due to the essentiality of silicon and calcium as accessory factors for cell activity stimulation.

Keywords
Glass ceramics; Cell culture; Apatite; Wollastonite; Cell proliferation
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In vitro study of the proliferation and growth of human bone marrow cells on apatite–wollastonite-2M glass ceramics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 6, June 2010, Pages 2254–2263
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us