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Application of metagenomic techniques in mining enzymes from microbial communities for biofuel synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
14315 1208 2012 10 PDF Available
Title
Application of metagenomic techniques in mining enzymes from microbial communities for biofuel synthesis
Abstract

Feedstock for biofuel synthesis is transitioning to lignocelluosic biomass to address criticism over competition between first generation biofuels and food production. As microbial catalysis is increasingly applied for the conversion of biomass to biofuels, increased import has been placed on the development of novel enzymes. With revolutionary advances in sequencer technology and metagenomic sequencing, mining enzymes from microbial communities for biofuel synthesis is becoming more and more practical. The present article highlights the latest research progress on the special characteristics of metagenomic sequencing, which has been a powerful tool for new enzyme discovery and gene functional analysis in the biomass energy field. Critical enzymes recently developed for the pretreatment and conversion of lignocellulosic materials are evaluated with respect to their activity and stability, with additional explorations into xylanase, laccase, amylase, chitinase, and lipolytic biocatalysts for other biomass feedstocks.

Keywords
Metagenomic sequencing; Biofuel synthesis; Critical enzymes; Genetic modification; Biomass
First Page Preview
Application of metagenomic techniques in mining enzymes from microbial communities for biofuel synthesis
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 30, Issue 4, July–August 2012, Pages 920–929
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering