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Biomimetic apatite formation on calcium phosphate-coated titanium in Dulbecco’s phosphate-buffered saline solution containing CaCl2 with and without fibronectin

Paper ID Volume ID Publish Year Pages File Format Full-Text
1433 83 2010 8 PDF Available
Title
Biomimetic apatite formation on calcium phosphate-coated titanium in Dulbecco’s phosphate-buffered saline solution containing CaCl2 with and without fibronectin
Abstract

Calcium phosphate (CaP) thin films with different degrees of crystallinity were coated on the surfaces of commercially pure titanium by electron beam evaporation. The details of apatite nucleation and growth on the coating layer were investigated in Dulbecco’s phosphate-buffered saline solutions containing calcium chloride (DPBS) or DPBS with fibronectin (DPBSF). The surfaces of the samples were examined by field emission scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The concentrations of fibronectin and calcium ions (Ca2+) were monitored by the bicinchoninic acid method (BCA) and use of a calcium assay kit (DICA-500), respectively. Apatite initially formed at the fastest rate on the CaP-coated samples with the lowest degree of crystallinity and reached the maximum Ca2+ concentration after immersion in DPBS solution for 15 min. After 15 min the concentration of Ca2+ decreased with the growth of apatite on the coating layers. For all the samples the maximum Ca2+ concentration in the DPBS solutions decreased with increasing crystallinity and immersion time to reach the maximum concentration increased. The presence of fibronectin in the DPBS solutions delayed the formation and affected the morphology of the apatite. Fibronectin incorporated into apatite deposited on the surface of titanium did not affect its biological activity in terms of promoting osteoblast adhesion.

Keywords
Calcium phosphate film; E-beam evaporation; Fibronectin; Biomimetic; Apatite
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Biomimetic apatite formation on calcium phosphate-coated titanium in Dulbecco’s phosphate-buffered saline solution containing CaCl2 with and without fibronectin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 6, June 2010, Pages 2274–2281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us