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Genome-scale genetic engineering in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
14343 1210 2013 7 PDF Available
Title
Genome-scale genetic engineering in Escherichia coli
Abstract

Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli as well as multiplex automated genome engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties.

Keywords
Genome engineering; λ Red recombination; Multiplex automated genome engineering
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Genome-scale genetic engineering in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 31, Issue 6, 1 November 2013, Pages 804–810
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us