fulltext.study @t Gmail

Production of bulk chemicals via novel metabolic pathways in microorganisms

Paper ID Volume ID Publish Year Pages File Format Full-Text
14353 1210 2013 11 PDF Available
Title
Production of bulk chemicals via novel metabolic pathways in microorganisms
Abstract

Metabolic engineering has been playing important roles in developing high performance microorganisms capable of producing various chemicals and materials from renewable biomass in a sustainable manner. Synthetic and systems biology are also contributing significantly to the creation of novel pathways and the whole cell-wide optimization of metabolic performance, respectively. In order to expand the spectrum of chemicals that can be produced biotechnologically, it is necessary to broaden the metabolic capacities of microorganisms. Expanding the metabolic pathways for biosynthesizing the target chemicals requires not only the enumeration of a series of known enzymes, but also the identification of biochemical gaps whose corresponding enzymes might not actually exist in nature; this issue is the focus of this paper. First, pathway prediction tools, effectively combining reactions that lead to the production of a target chemical, are analyzed in terms of logics representing chemical information, and designing and ranking the proposed metabolic pathways. Then, several approaches for potentially filling in the gaps of the novel metabolic pathway are suggested along with relevant examples, including the use of promiscuous enzymes that flexibly utilize different substrates, design of novel enzymes for non-natural reactions, and exploration of hypothetical proteins. Finally, strain optimization by systems metabolic engineering in the context of novel metabolic pathways constructed is briefly described. It is hoped that this review paper will provide logical ways of efficiently utilizing ‘big’ biological data to design and develop novel metabolic pathways for the production of various bulk chemicals that are currently produced from fossil resources.

► In silico prediction tools are important in designing novel metabolic pathway. ► Enzyme promiscuity and modification are considered to fill in the biochemical gap. ► Genome mining is an alternative method to fill in the biochemical gap. ► Systems metabolic engineering is needed for strain optimization.

Keywords
1,4-BDO, 1,4-butanediol; 3HP, 3-hydroxypropionic acid; 4HB-CoA, 4-hydroxybutyryl-CoA; ADH, alcohol dehydrogenase; BEM, bond and electron matrix; BNICE, Biochemical Network Integrated Computational Explorer; EC, Enzyme Commission; GOLD, Genomes OnLine Data
First Page Preview
Production of bulk chemicals via novel metabolic pathways in microorganisms
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 31, Issue 6, 1 November 2013, Pages 925–935
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us