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Classifying DNA assembly protocols for devising cellular architectures

Paper ID Volume ID Publish Year Pages File Format Full-Text
14373 1213 2011 8 PDF Available
Title
Classifying DNA assembly protocols for devising cellular architectures
Abstract

DNA assembly is one of the most fundamental techniques in synthetic biology. Efficient methods can turn traditional DNA cloning into time-saving and higher efficiency practice, which is a foundation to accomplish the dreams of synthetic biologists for devising cellular architectures, reprogramming cellular behaviors, or creating synthetic cells. In this review, typical strategies of DNA assembly are discussed with special emphasis on the assembly of long and multiple DNA fragments into intact plasmids or assembled compositions. Constructively, all reported strategies were categorized into in vivo and in vitro types, and protocols are presented in a functional and practice-oriented way in order to portray the general nature of DNA assembly applications. Significantly, a five-step blueprint is proposed for devising cell architectures that produce valuable chemicals.

Keywords
DNA assembly; In vivo; In vitro; Synthetic biology; Metabolic engineering; Cellular architecture
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Classifying DNA assembly protocols for devising cellular architectures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 29, Issue 1, January–February 2011, Pages 156–163
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us