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Recent advances in bioprocessing application of membrane chromatography

Paper ID Volume ID Publish Year Pages File Format Full-Text
14387 1215 2013 16 PDF Available
Title
Recent advances in bioprocessing application of membrane chromatography
Abstract

Compared to traditional chromatography using resins in packed-bed columns, membrane chromatography is a relatively new and immature bioseparation technology based on the integration of membrane filtration and liquid chromatography into a single-stage operation. Over the past decades, advances in membrane chemistry have yielded novel membrane devices with high binding capacities and improved mass transfer properties, significantly increasing the bioprocessing efficiency for purification of biomolecules. Due to the disposable nature, low buffer consumption, and reduced equipment costs, membrane chromatography can significantly reduce downstream bioprocessing costs. In this review, we discuss technological merits and disadvantages associated with membrane chromatography as well as recent bioseparation applications with a particular attention on purification of large biomolecules.

Keywords
AcMNVP, Autographa californica multicapsid nucleopolyhedrovirus; AeDNV, Aedes aegypti densonucleosis virus; AGM, agmatine; BSA, bovine serum albumin; BUDGE, 1,4-butanediol diglycidyl ether; C, carboxyl weak cation-exchanger; CHO, Chinese hamster ovarian c
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 31, Issue 4, July–August 2013, Pages 450–465
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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