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Nitrile hydratases (NHases): At the interface of academia and industry

Paper ID Volume ID Publish Year Pages File Format Full-Text
14419 1219 2010 17 PDF Available
Title
Nitrile hydratases (NHases): At the interface of academia and industry
Abstract

Nitrile hydratase (NHase, EC 4.2.1.84) is one of the key enzymes of nitrile metabolism in a large number of microbes that catalyses the hydration of nitriles to corresponding amides, and has been successfully adopted in chemical industry for production of acrylamide, nicotinamide and 5-cyanovaleramide. However, NHase is still under active consideration of enzymologists to expand its potential for synthesis of various amides. Most of the NHases have been reported for their limited substrates acceptability, low enantioselectivity and thermostability and therefore a considerable improvement is required for developing as robust biocatalyst for synthesis of a range of organic amides. Studies on biochemical properties, gene configuration, active-site chemical models and site-directed mutagenesis have given the insight into the structural and functional characteristics of NHase. Keeping in view, the present review critically describes the available information on natural sources (based on activity and phylogenetic analysis), biochemical properties, catalysis–structure relationship, molecular expression and potential applications of this enzyme.

Keywords
Nitrile hydratases (NHases); Nitrile metabolism; Amides synthesis; Biocatalysis; Enzyme engineering; Metalloenzyme; Structure–function relation; Gene expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 28, Issue 6, November–December 2010, Pages 725–741
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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Full-text PDF Download
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