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A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains

Paper ID Volume ID Publish Year Pages File Format Full-Text
14468 1223 2012 10 PDF Available
Title
A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains
Abstract

Sucrose has economic and environmental advantages over glucose as a feedstock for bioprocesses. E. coli is widely used in industry, but the majority of current industrial E. coli strains cannot utilize sucrose. Previous attempts to transfer sucrose catabolic capabilities into non-sucrose-utilizing strains have met with limited success due to low growth rates on sucrose and phenotypic instability of the engineered strains. To address these problems, we developed a transferrable sucrose utilization cassette which confers efficient sucrose catabolism when integrated onto the E. coli chromosome. The cassette was based on the csc genes from E. coli W, a strain which grows very quickly on sucrose. Both plasmid-borne expression and chromosomal integration of a repressor-less sucrose utilizing cassette were investigated in E. coli strains K-12, B and C. In contrast to previous studies, strains harboring chromosomal cassettes could grow at the same rate as they do on glucose. Interestingly, we also discovered that spontaneous chromosomal integration of the csc genes was required to allow efficient growth from plasmid-transformed strains. The ability to engineer industrial strains for efficient sucrose utilization will allow substitution of sucrose for glucose in industrial fermentations. This will encourage the use of sucrose as a carbon source and assist in transition of our petrochemical-based economy to a bio-based economy.

Keywords
csc, chromosomally-encoded sucrose catabolism; KI, knock-in; FRT, flippase recognition target; PEP, phosphoenolpyruvate; PTS, PEP-dependent sugar:phosphotransferase system; OD, optical density; IPTG, isopropyl β-d-1-thiogalactopyranosideTransferrable sucr
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A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 30, Issue 5, September–October 2012, Pages 1001–1010
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us