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Mammalian cell protein expression for biopharmaceutical production

Paper ID Volume ID Publish Year Pages File Format Full-Text
14481 1223 2012 13 PDF Available
Title
Mammalian cell protein expression for biopharmaceutical production
Abstract

Mammalian cell expression has become the dominant recombinant protein production system for clinical applications because of its capacity for post-translational modification and human protein-like molecular structure assembly. While expression and production have been fully developed and Chinese hamster ovary cells are used for the majority of products both on the market and in clinical development, significant progresses in developing and engineering new cell lines, introducing novel genetic mechanisms in expression, gene silencing, and gene targeting, have been reported in the last several years. With the latest analytical methods development, more attention is being devoted towards product quality including glycol profiling, which leads to better understanding the impact of culture condition during production. Additionally, transient gene expression technology platform plays more important role in biopharmaceutical early development stages. This review focused on the latest advancements in the field, especially in active areas such as expression systems, glycosylation impact factors, and transient gene expression.

Keywords
6-TG, 6-thioguanine; ACE, Artificial Chromosome Expression; ADCC, Antibody-dependent Cellular Cytotoxicity; BGH, Bovine Growth Hormone; BHK, Baby Hamster Kidney; CHO, Chinese Hamster Ovary; CLL, Chronic Lymphocytic Leukemia; CMV, Cytomegalovirus; CTE, Con
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 30, Issue 5, September–October 2012, Pages 1158–1170
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us