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Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion

Paper ID Volume ID Publish Year Pages File Format Full-Text
14486 1224 2008 17 PDF Available
Title
Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion
Abstract

Reversed micelle solvents represent nanometer-sized aqueous droplets stabilized by surfactants inside the bulk organic solvents. The aqueous cores can host various hydrophilic solutes, including bioactive substances thus revealing a challenge to the biotechnology's needs of the safe media for bioseparations and bioconversions. This review discusses the structure and the properties of reversed micelle solvents in view of the parameters that can be easily operated in technology to achieve safe liquid–liquid extraction of proteins/enzymes or bioconversion of hydrophobic substrates. The paper highlights the importance of how the reversed micelle microenvironment should be arranged with respect to the preservation of the activity of the enzyme as target product or biocatalyst. The main aspects are demonstrated with own experimental results on α-amylase purification and lipase-catalyzed esterification using cationic reversed micelle solvents. The trials of performing continuous processes involving reversed micellar separation and reaction media are also reviewed and the current problems are addressed.

Keywords
Reversed micelle solvent; Reversed micelle extraction; Enzyme purification; α-Amylase; Heterogeneous biocatalysis; Lipase; Cationic surfactant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 26, Issue 6, November–December 2008, Pages 516–532
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us