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Strategies for accessing soil metagenome for desired applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
14490 1224 2008 15 PDF Available
Title
Strategies for accessing soil metagenome for desired applications
Abstract

Most of the microorganisms in nature are inaccessible as they are uncultivable in the laboratory. Metagenomic approaches promise the accessibility of the genetic resources and their potential applications. Genetic resources from terrestrial environments can be accessed by exploring the soil metagenome. Soil metagenomic analyses are usually initiated by the isolation of environmental DNAs. Several methods have been described for the direct isolation of environmental DNAs from soil and sediments. Application of metagenomics largely depends on the construction of genomic DNA libraries and subsequent high-throughput sequencing or library screening. Thus, obtaining large quantities of pure cloneable DNA from the environment is a prerequisite. This review discusses the recent developments related to efficient extraction and purification of soil metagenome highlighting the considerations for various metagenomic applications.

Keywords
Metagenomics; Microbial diversity; Mobile genomics; Single cell genomics; Soil DNA; Sub-metagenomic library
First Page Preview
Strategies for accessing soil metagenome for desired applications
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 26, Issue 6, November–December 2008, Pages 576–590
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering