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The role of activated charcoal in plant tissue culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
14493 1224 2008 14 PDF Available
Title
The role of activated charcoal in plant tissue culture
Abstract

Activated charcoal has a very fine network of pores with large inner surface area on which many substances can be adsorbed. Activated charcoal is often used in tissue culture to improve cell growth and development. It plays a critical role in micropropagation, orchid seed germination, somatic embryogenesis, anther culture, synthetic seed production, protoplast culture, rooting, stem elongation, bulb formation etc. The promotary effects of AC on morphogenesis may be mainly due to its irreversible adsorption of inhibitory compounds in the culture medium and substancially decreasing the toxic metabolites, phenolic exudation and brown exudate accumulation. In addition to this activated charcoal is involved in a number of stimulatory and inhibitory activities including the release of substances naturally present in AC which promote growth, alteration and darkening of culture media, and adsorption of vitamins, metal ions and plant growth regulators, including abscisic acid and gaseous ethylene. The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants. This review focuses on the various roles of activated charcoal in plant tissue culture and the recent developments in this area.

Keywords
ABA, Abscisic acid; AC, Activated charcoal; BA, 6-Benzyl aminopurine; BP, Banana pulp; CH, Casein hydrolysate; CW, Coconut water; 2,4-D, 2, 4-Dichlorophenoxyacetic acid; GD, Gresshoff and Doy medium; IAA, Indole 3-acetic acid; IBA, Indole-3-butyric acid;
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The role of activated charcoal in plant tissue culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 26, Issue 6, November–December 2008, Pages 618–631
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us