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A decade of improvements in quantification of gene expression and internal standard selection

Paper ID Volume ID Publish Year Pages File Format Full-Text
14495 1225 2009 11 PDF Available
Title
A decade of improvements in quantification of gene expression and internal standard selection
Abstract

Major improvements have been made in mRNA quantification and internal standard selection over the last decade. Our aim in this paper is to present the main developments that are of interest for practical laboratory work, contrasting the situation as it is now with the one of ten years ago, and presenting some excellent examples of what can be done today. Specifically, we will mainly discuss Real-Time RT-PCR major improvements that have been performed in the following areas: the most commonly used quantification techniques, the mathematical and software tools created to help researchers in their work on internal standard selection, the availability of detection chemistries and technical information and of commercial tools and services. In addition to mRNA quantification, we will also discuss some aspects of non-coding RNA and protein quantification. In addition to technical improvements, the development of international cooperation and the creation of technical databases are likely to represent a major tool for the future in the standardization of gene expression quantification.

Keywords
Housekeeping gene; Reference gene selection; Real-Time RT-PCR; mRNA quantification; Software tool; Reference protein; Protein quantification; Non-coding RNA
First Page Preview
A decade of improvements in quantification of gene expression and internal standard selection
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 4, July–August 2009, Pages 323–333
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering