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Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development

Paper ID Volume ID Publish Year Pages File Format Full-Text
14536 1227 2012 16 PDF Available
Title
Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development
Abstract

In the present review we discuss strategies that have been used for heterologous gene expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since the growth of S2 cells is not dependent on anchorage to solid substrates, these cells can be easily cultured in suspension in large volumes. The factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. Gene expression, cell metabolism, culture medium formulation and parameters involved in cellular respiration are particularly emphasized. The experience of the authors with the successful expression of a biologically functional protein, the rabies virus glycoprotein (RVGP), by recombinant S2 cells is presented in the topics covered.

Keywords
Drosophila melanogaster; Schneider S2 cells; Gene expression; Heterologous genes; Recombinant proteins; Rabies virus glycoprotein; Bioprocess
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Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 30, Issue 3, May–June 2012, Pages 613–628
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us