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Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

Paper ID Volume ID Publish Year Pages File Format Full-Text
14547 1227 2012 16 PDF Available
Title
Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks
Abstract

The baculovirus expression vector system (BEVS) is a versatile and powerful platform for protein expression in insect cells. With the ability to approach similar post-translational modifications as in mammalian cells, the BEVS offers a number of advantages including high levels of expression as well as an inherent safety during manufacture and of the final product. Many BEVS products include proteins and protein complexes that require expression from more than one gene. This review examines the expression strategies that have been used to this end and focuses on the distinguishing features between those that make use of single polycistronic baculovirus (co-expression) and those that use multiple monocistronic baculoviruses (co-infection). Three major areas in which researchers have been able to take advantage of co-expression/co-infection are addressed, including compound structure-function studies, insect cell functionality augmentation, and VLP production. The core of the review discusses the parameters of interest for co-infection and co-expression with time of infection (TOI) and multiplicity of infection (MOI) highlighted for the former and the choice of promoter for the latter. In addition, an overview of modeling approaches is presented, with a suggested trajectory for future exploration. The review concludes with an examination of the gaps that still remain in co-expression/co-infection knowledge and practice.

Keywords
AAV, adeno-associated virus; AcMNPV, Autographa californica multiple nucleopolyhedrovirus; ATP, adenosine triphosphate; BEVS, baculovirus expression vector system; BiP, binding immunoglobin protein; Cap, capsid; CLP, core-like particle; CMP-SAS, CMP-siali
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Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 30, Issue 3, May–June 2012, Pages 766–781
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us