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Deep insights into Dictyocaulus viviparus transcriptomes provides unique prospects for new drug targets and disease intervention

Paper ID Volume ID Publish Year Pages File Format Full-Text
14612 1233 2011 11 PDF Available
Title
Deep insights into Dictyocaulus viviparus transcriptomes provides unique prospects for new drug targets and disease intervention
Abstract

The lungworm, Dictyocaulus viviparus, causes parasitic bronchitis in cattle, and is responsible for substantial economic losses in temperate regions of the world. Here, we undertake the first large-scale exploration of available transcriptomic data for this lungworm, examine differences in transcription between different stages/both genders and identify and prioritize essential molecules linked to fundamental metabolic pathways, which could represent novel drug targets. Approximately 3 million expressed sequence tags (ESTs), generated by 454 sequencing from third-stage larvae (L3s) as well as adult females and males of D. viviparus, were assembled and annotated. The assembly of these sequences yielded ~ 61,000 contigs, of which relatively large proportions encoded collagens (4.3%), ubiquitins (2.1%) and serine/threonine protein kinases (1.9%). Subtractive analysis in silico identified 6928 nucleotide sequences as being uniquely transcribed in L3, and 5203 and 7889 transcripts as being exclusive to the adult female and male, respectively. Most peptides predicted from the conceptual translations were nucleoplasmins (L3), serine/threonine protein kinases (female) and major sperm proteins (male). Additional analyses allowed the prediction of three drug target candidates, whose Caenorhabditis elegans homologues were linked to a lethal RNA interference phenotype. This detailed exploration, combined with future transcriptomic sequencing of all developmental stages of D. viviparus, will facilitate future investigations of the molecular biology of this parasitic nematode as well as genomic sequencing. These advances will underpin the discovery of new drug and/or vaccine targets, focused on biotechnological outcomes.

Keywords
Dictyocaulus viviparus; Bovine lungworm; Next-generation sequencing; Bioinformatics; Transcriptome; Ancylostoma-secreted proteins; Drug target prediction
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Deep insights into Dictyocaulus viviparus transcriptomes provides unique prospects for new drug targets and disease intervention
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 29, Issue 3, May–June 2011, Pages 261–271
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us