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Isolation and affinity maturation of hapten-specific antibodies

Paper ID Volume ID Publish Year Pages File Format Full-Text
14635 1236 2007 20 PDF Available
Title
Isolation and affinity maturation of hapten-specific antibodies
Abstract

More and more recombinant antibodies specific for haptens such as drugs of abuse, dyes and pesticides are being isolated from antibody libraries. Thereby isolated antibodies tend to possess lower affinity than their parental, full-size counterparts, and therefore the isolation techniques must be optimized or the antibody genes must be affinity-matured in order to reach high affinities and specificities required for practical applications. Several strategies have been explored to obtain high-affinity recombinant antibodies from antibody libraries: At the selection level, biopanning optimization can be performed through elution with free hapten, analogue pre-incubation and subtractive panning. At the mutagenesis level, techniques such as random mutagenesis, bacterial mutator strains passaging, site-directed mutagenesis, mutational hotspots targeting, parsimonious mutagenesis, antibody shuffling (chain, DNA and staggered extension process) have been used with various degrees of success to affinity mature or modify hapten-specific antibodies. These techniques are reviewed, illustrated and compared.

Keywords
Affinity maturation; Antibody libraries; Biopanning; Hapten; Mutagenesis; Phage display; Recombinant antibody; Ribosome display
First Page Preview
Isolation and affinity maturation of hapten-specific antibodies
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 25, Issue 4, July–August 2007, Pages 333–352
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering