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Fungal nitrilases as biocatalysts: Recent developments

Paper ID Volume ID Publish Year Pages File Format Full-Text
14642 1237 2009 10 PDF Available
Title
Fungal nitrilases as biocatalysts: Recent developments
Abstract

Of the numerous putative fungal nitrilases available from protein databases only a few enzymes were purified and characterized. The purified nitrilases from Fusarium solani, Fusarium oxysporum f. sp. melonis and Aspergillus niger share a preference for (hetero)aromatic nitriles, temperature optima between 40 and 50 °C and pH optima in the slightly alkaline region. On the other hand, they differ in their chemoselectivity, i.e. their tendency to produce amides as by-products. The production of fungal nitrilases is increased by up to three orders of magnitude on the addition of 2-cyanopyridine to the culture media. The whole-cell and subcellular biocatalysts were immobilized by various methods (LentiKats®; adsorption on hydrophobic or ion exchange resins; cross-linked enzyme aggregates). Operational stability was examined using continuous stirred membrane bioreactors. Fungal nitrilases appear promising for biocatalytic applications and biodegradation of nitrile environmental contaminants.

Keywords
Nitrilase; Filamentous fungi; Aspergillus; Fusarium; Nitrile biotransformation; Nitrile pollutant biodegradation; Immobilized biocatalysts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 6, November–December 2009, Pages 661–670
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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