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The myth of plant transformation

Paper ID Volume ID Publish Year Pages File Format Full-Text
14650 1237 2009 11 PDF Available
Title
The myth of plant transformation
Abstract

Technology development is innovative to many aspects of basic and applied plant transgenic science. Plant genetic engineering has opened new avenues to modify crops, and provided new solutions to solve specific needs. Development of procedures in cell biology to regenerate plants from single cells or organized tissue, and the discovery of novel techniques to transfer genes to plant cells provided the prerequisite for the practical use of genetic engineering in crop modification and improvement. Plant transformation technology has become an adaptable platform for cultivar improvement as well as for studying gene function in plants. This success represents the climax of years of efforts in tissue culture improvement, in transformation techniques and in genetic engineering. Plant transformation vectors and methodologies have been improved to increase the efficiency of transformation and to achieve stable expression of transgenes in plants. This review provides a comprehensive discussion of important issues related to plant transformation as well as advances made in transformation techniques during three decades.

Keywords
Transformation; Agrobacterium; Liposomes; Biolistic gun; Infiltration; Silicon carbide; Microinjection
First Page Preview
The myth of plant transformation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 6, November–December 2009, Pages 753–763
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering