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Structural and catalytic polymorphism of human enzymes: Novel potential platforms for biomedical diagnostics

Paper ID Volume ID Publish Year Pages File Format Full-Text
14665 1237 2009 15 PDF Available
Title
Structural and catalytic polymorphism of human enzymes: Novel potential platforms for biomedical diagnostics
Abstract

This review focuses on some intermediate results on the path from the gene and enzyme structure to physiological responses and personalised medicine. Bioinformatics of genetic and protein-structural polymorphisms, theoretical methods of predicting the influence of single amino acid substitutions on the structure and catalytic activity of enzymes are considered. For a large group of enzymes, interrelations between genetic modifications, structural changes of the proteins and the detected physiological and clinical manifestations are discussed. In this respect, highly productive techniques to determine the catalytic activity of an enzyme as well as non-invasive proteomic approaches are of particular interest. A non-invasive proteomic analysis using mass-spectrometric protein identification of human exhaled breath condensate and tear fluids has been chosen.

Keywords
Enzymes; Single nucleotide polymorphism; Proteomics; Protein mass spectrometry; Human exhaled breath condensate; High-throughput enzyme assay; Tear fluid; Aqueous humour
First Page Preview
Structural and catalytic polymorphism of human enzymes: Novel potential platforms for biomedical diagnostics
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 6, November–December 2009, Pages 945–959
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering