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Genetic engineering of antibiotic biosynthesis for the generation of new aminocoumarins

Paper ID Volume ID Publish Year Pages File Format Full-Text
14670 1237 2009 9 PDF Available
Title
Genetic engineering of antibiotic biosynthesis for the generation of new aminocoumarins
Abstract

The aminocoumarin antibiotics novobiocin, clorobiocin and coumermycin A1 are inhibitors of gyrase and highly effective antibacterial agents. Their biosynthetic gene clusters have been cloned from the respective Streptomyces producer strains, and the function of nearly all genes contained therein has been elucidated by genetic and biochemical methods. Efficient methods have been developed for the genetic manipulation and the heterologous expression of the clusters, and more than 100 new derivatives of these antibiotics have been generated by metabolic engineering, mutasynthesis and chemoenzymatic synthesis, providing a model for the power of genetic and genomic methods for the generation of new bioactive compounds.

Keywords
Antibiotics; Gyrase inhibitors; Aminocoumarin; Novobiocin; Clorobiocin; Coumermycin; Gene cluster; Combinatorial biosynthesis
First Page Preview
Genetic engineering of antibiotic biosynthesis for the generation of new aminocoumarins
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 6, November–December 2009, Pages 1006–1014
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering