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25 years of recombinant proteins from reactor-grown cells — Where do we go from here?

Paper ID Volume ID Publish Year Pages File Format Full-Text
14672 1237 2009 5 PDF Available
Title
25 years of recombinant proteins from reactor-grown cells — Where do we go from here?
Abstract

The purpose of this review is to describe the current status and to highlight several emerging trends in the manufacture of recombinant therapeutic proteins in cultivated mammalian cells, focusing on Chinese hamster ovary cells as the major production host. Over the past 25 years, specific and volumetric productivities for recombinant cell lines have increased about 20-fold as the result of improvements in media and bioprocess design. Future yield increases are expected to come from further developments in gene delivery and genetic selection for more efficient recovery of high-producing cell lines and in high-throughput cultivation systems to simplify medium design and bioprocess development. Other emerging trends in protein manufacturing that are discussed include the use of disposal bioreactors and transient gene expression. We specifically highlight current research in our own laboratories.

Keywords
CHO; Bioreactor; Orbital shaking; Recombinant protein; Cell culture medium; Transfection; Transient gene expression; Disposables
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25 years of recombinant proteins from reactor-grown cells — Where do we go from here?
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 27, Issue 6, November–December 2009, Pages 1023–1027
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us