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Downstream processing of oncoretroviral and lentiviral gene therapy vectors

Paper ID Volume ID Publish Year Pages File Format Full-Text
14696 1240 2006 17 PDF Available
Title
Downstream processing of oncoretroviral and lentiviral gene therapy vectors
Abstract

Retroviral vectors from both oncoretroviral and lentiviral origins have a great potential as gene delivery vehicles. A number of research groups have devoted considerable effort to the development of large-scale production strategies for retroviral vectors. However, the manufacturing of clinical-grade vectors for gene therapy, especially for in vivo applications, additionally requires scaleable purification strategies to remove the contaminants present in the harvested supernatants while preserving the functionality of the vectors. In this article, we review recent advances made in the field of downstream processing of retroviral vectors. The methods currently described in the literature for clarification, concentration and purification of retroviral vectors will be presented, with special emphasis on novel chromatography methods that open up the possibility to selectively and efficiently purify retroviruses on a large-scale. Problems associated with stability and quantification of retroviral particles will be outlined and future challenges will be discussed.

Keywords
Oncovirus; Retrovirus; Lentivirus; Purification; Clarification; Concentration; Density gradient; Rate zonal ultracentrifugation; Affinity chromatography; Heparin
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Downstream processing of oncoretroviral and lentiviral gene therapy vectors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 24, Issue 3, May–June 2006, Pages 321–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us