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Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest

Paper ID Volume ID Publish Year Pages File Format Full-Text
14709 1241 2010 10 PDF Available
Title
Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest
Abstract

Methods to increase the production of recombinant proteins in mammalian cell cultures have been developed which reduce in-culture growth through prohibiting progression of the cell cycle. This arrest increases the proportion of cells in the G1-phase of the cell cycle, and subsequently increases their specific productivity (QP). Through careful balancing of the decreased growth rate with an increased QP, multi-fold increases in recombinant protein yield can be achieved.Induction of this arrest can occur through the addition of cytostatic chemical agents (NaBu or DMSO), over-expression of cell cycle inhibitory proteins (p21Cip1 or p27Kip1), or through control of the culture environment (mild hypothermic temperatures). While all these controlled proliferation strategies have been developed independently, they all aim to control cell proliferation through a shared mechanism; the direct or indirect up-regulation of members of the cyclin-dependent kinase inhibitor (CKI) family.This review highlights the shared biology of these three controlled proliferation strategies, and their contribution towards bioprocess development for recombinant production from animal cell cultures.

Keywords
Controlled proliferation; Hypothermic growth; Sodium butyrate; CHO; Recombinant protein; CDK; CKI
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Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 28, Issue 3, May–June 2010, Pages 385–394
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us