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Enzymatic microreactors in chemical analysis and kinetic studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
14745 1245 2006 16 PDF Available
Title
Enzymatic microreactors in chemical analysis and kinetic studies
Abstract

The fields of application of microreactors are becoming wider every year. A considerable number of papers have been published recently reporting successful application of enzymatic microreactors in chemistry and biochemistry. Most are devices with enzymes immobilized on beads or walls of microfluidic channels, whilst some use dissolved enzymes to run a reaction in the microfluidic system. Apart from model systems, mostly with glucose oxidase, horseradish peroxidase and alkaline phosphatase, the principal fields of application of microreactors are tryptic digestion of proteins and polymerase chain reaction in automated analyses of proteomic and genetic material, respectively. Enzymatic microreactors also facilitate characterization of enzyme activity as a function of substrate concentration, and enable fast screening of new biocatalysts and their substrates. They may constitute key parts of lab-on-a-chip and μTAS, assisting the analysis of biomolecules. This review provides systematic coverage of examples of reports on enzymatic microreactors published recently, as well as relevant older papers.

Keywords
Characterization of proteins and DNA; Enzyme immobilization; Lab-on-a-chip; Microfluidic systems; Microreactors
First Page Preview
Enzymatic microreactors in chemical analysis and kinetic studies
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 24, Issue 1, January–February 2006, Pages 42–57
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering