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Toward practical, DNA-based diagnostic methods for parasitic nematodes of livestock — Bionomic and biotechnological implications

Paper ID Volume ID Publish Year Pages File Format Full-Text
14800 1252 2008 10 PDF Available
Title
Toward practical, DNA-based diagnostic methods for parasitic nematodes of livestock — Bionomic and biotechnological implications
Abstract

Parasitic nematodes of livestock have a major economic impact worldwide. In spite of the health problems caused by nematodes and advances toward the development of vaccines and new therapeutic agents against some of them, relatively limited attention has been paid to the need for improved, practical methods of diagnosis. Accurate diagnosis and genetic characterization of parasitic nematodes of livestock are central to their effective control, particularly given the current, serious problems with anthelmintic resistance in nematode populations. Traditional diagnostic techniques have considerable limitations, and there have been some advances toward the development of molecular-diagnostic tools. This article provides a brief account of the significance of parasitic nematodes (order Strongylida), reviews the techniques that have been evaluated or used for diagnosis and describes developments in polymerase chain reaction (PCR)-based methods for the specific diagnosis of nematode infection/s and the genetic characterisation of the causative agents. The advances made in recent years provide a solid foundation for the development of practical, highly sensitive and specific diagnostic tools for epidemiological investigations and for use in control programmes.

Keywords
Livestock; Nematoda; Strongylida; Specific diagnosis of infection; Genetic variation; Polymerase chain reaction (PCR); Mutation scanning; Genetic markers; Internal transcribed spacers of nuclear ribosomal DNA
First Page Preview
Toward practical, DNA-based diagnostic methods for parasitic nematodes of livestock — Bionomic and biotechnological implications
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 26, Issue 4, July–August 2008, Pages 325–334
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering