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Critical assessment of current adeno-associated viral vector production and quantification methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
14812 1253 2008 16 PDF Available
Title
Critical assessment of current adeno-associated viral vector production and quantification methods
Abstract

Adeno-associated viral vectors have emerged as one of the most studied vectors for gene therapy. Numerous production methods have been described, each with its advantages and disadvantages. A challenge in assessing the current state of the art exists in comparing yields from one production system to the next due to the wide variety of quantification techniques. In this review, AAV vector production methods are summarized and the yields of the different processes are standardized to the number of harvested cells. Titers are further streamlined into five categories: transduction units, enhanced transduction units, infectious particles, DNase-resistant particles and total particles, and the importance of each type of measure is discussed.

Keywords
Review; Production; Quantification; Adeno-associated virus; ELISA; PCR; Infectious assay; Replication assay; Transduction assay
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Critical assessment of current adeno-associated viral vector production and quantification methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 26, Issue 1, January–February 2008, Pages 73–88
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us