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Synthesis, regulation and production of urokinase using mammalian cell culture: A comprehensive review

Paper ID Volume ID Publish Year Pages File Format Full-Text
14828 1257 2006 15 PDF Available
Title
Synthesis, regulation and production of urokinase using mammalian cell culture: A comprehensive review
Abstract

Urokinase, a serine protease, catalyzes the conversion of plasminogen to plasmin, which is responsible for dissolution of clots in blood vessels. It is an important drug for treatment of thromboembolic disease. Production of urokinase by mammalian cell culture has the following important steps: synthesis, regulation and secretion. Production and accumulation of this product in a bioreactor is a real challenge for biochemical engineers. Considerable information at molecular level needs to be understood for production of urokinase in order to correlate different parameters, which in turn can maximize the productivity. This information will be highlighted in this review. Moreover, urokinase production is a product-inhibited process. Therefore, in situ urokinase separation strategy is required to operate a bioreactor at its maximum urokinase formation rate. Integrated urokinase production and isolation processes developed recently will also be discussed briefly in this review.

Keywords
Ras ERK; Ras-extracellular signal-related protein kinase; Rac JNK; Rak-c-Jun N-terminal kinase; PLD; Phospholipase D; MEK; Mitogen-activated protein Extracellular Kinase; MAPK; Mtiogen activated Protein kinase; PKC-ERK; Protein kinase C-extracellular sign
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Synthesis, regulation and production of urokinase using mammalian cell culture: A comprehensive review
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 24, Issue 5, September–October 2006, Pages 514–528
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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