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Packed-bed bioreactors for mammalian cell culture: Bioprocess and biomedical applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
14845 1264 2007 12 PDF Available
Title
Packed-bed bioreactors for mammalian cell culture: Bioprocess and biomedical applications
Abstract

This article describes the development history of packed-bed bioreactors (PBRs) used for the culture of mammalian cells. It further reviews the current applications of PBRs and discusses the steps forward in the development of these systems for bioprocess and biomedical applications. The latest generation of PBRs used in bioprocess applications achieve very high cell densities (> 108 cells ml− 1) leading to outstandingly high volumetric productivity. However, a major bottleneck of such PBRs is their relatively small volume. The current maximal volume appears to be in the range of 10 to 30 l. A scale-up of more than 10-fold would be necessary for these PBRs to be used in production processes. In biomedical applications, PBRs have proved themselves as compact bioartificial organs, but their metabolic activity declines frequently within 1 to 2 weeks of operation. A main challenge in this field is to develop cell lines that grow consistently to high cell density in vitro and maintain a stable phenotype for a minimum of 1 to 2 months. Achieving this will greatly enhance the usefulness of PBR technology in clinical practice.

Keywords
Bioreactors; Mammalian cells; Packed-beds; Bioartificial organs
First Page Preview
Packed-bed bioreactors for mammalian cell culture: Bioprocess and biomedical applications
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 25, Issue 1, January–February 2007, Pages 45–56
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering