fulltext.study @t Gmail

A novel quantitative model of cell cycle progression based on cyclin-dependent kinases activity and population balances

Paper ID Volume ID Publish Year Pages File Format Full-Text
15041 1369 2015 13 PDF Available
Title
A novel quantitative model of cell cycle progression based on cyclin-dependent kinases activity and population balances
Abstract

•A novel model of cell cycle progression is proposed in this work.•The model is based on a population balance (PB) approach.•Cell phase transition is simulated by means of a suitable biochemical model.•Examples are discussed to illustrate the ability of the proposed model.

Cell cycle regulates proliferative cell capacity under normal or pathologic conditions, and in general it governs all in vivo/in vitro cell growth and proliferation processes. Mathematical simulation by means of reliable and predictive models represents an important tool to interpret experiment results, to facilitate the definition of the optimal operating conditions for in vitro cultivation, or to predict the effect of a specific drug in normal/pathologic mammalian cells. Along these lines, a novel model of cell cycle progression is proposed in this work. Specifically, it is based on a population balance (PB) approach that allows one to quantitatively describe cell cycle progression through the different phases experienced by each cell of the entire population during its own life. The transition between two consecutive cell cycle phases is simulated by taking advantage of the biochemical kinetic model developed by Gérard and Goldbeter (2009) which involves cyclin-dependent kinases (CDKs) whose regulation is achieved through a variety of mechanisms that include association with cyclins and protein inhibitors, phosphorylation–dephosphorylation, and cyclin synthesis or degradation. This biochemical model properly describes the entire cell cycle of mammalian cells by maintaining a sufficient level of detail useful to identify check point for transition and to estimate phase duration required by PB. Specific examples are discussed to illustrate the ability of the proposed model to simulate the effect of drugs for in vitro trials of interest in oncology, regenerative medicine and tissue engineering.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Population balance; Cell cycle; Modeling; Bioreactions; Kinetic parameters; Tissue cell culture
First Page Preview
A novel quantitative model of cell cycle progression based on cyclin-dependent kinases activity and population balances
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 55, April 2015, Pages 1–13
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us