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Gene expression regulation of the PF00480 or PF14340 domain proteins suggests their involvement in sulfur metabolism

Paper ID Volume ID Publish Year Pages File Format Full-Text
15071 1371 2014 7 PDF Available
Title
Gene expression regulation of the PF00480 or PF14340 domain proteins suggests their involvement in sulfur metabolism
Abstract

•We predict the involvement of poorly characterized proteins in sulfur metabolism.•The cysteine-dependent regulation was predicted for many genes.•The regulon contains subunits of the transporters of sulfur-containing compounds.•The regulated genes might have been horizontally transferred.•The results considerably extend studies of classic and Rho-dependent attenuations.

The paper studies proteins with domains PF00480 or PF14340, as well as some other poorly characterized proteins, encoded by genes associated with leader peptide genes containing a tract of cysteine codons. Such proteins are hypothetically regulated with cysteine-dependent transcription attenuation, namely the Rho-dependent or classic transcription attenuation. Cysteine is an important structural amino acid in various proteins and is required for synthesis of many sulfur-containing compounds, such as methionine, thiamine, glutathione, taurine and the lipoic acid. Earlier a few species of mycobacteria were predicted by the authors to have cysteine-dependent regulation of operons containing the cysK gene. In Escherichia coli this regulation is absent, and the same operon is regulated by the CysB transcription activator. The paper also studies Rho-dependent and classic transcription regulations in all annotated genes of mycobacteria available in GenBank and their orthologs in Actinomycetales. We predict regulations for many genes involved in sulfur metabolism and transport of sulfur-containing compounds; these regulations differ considerably among species. On the basis of predictions, we assign a putative role to proteins encoded by the regulated genes with unknown function, and also describe the structure of corresponding regulons, predict the lack of such regulations for many genes. Thus, all proteins with the uncharacterized Pfam domains PF14340 and PF00480, as well as some others, are predicted to be involved in sulfur metabolism. We also surmise the affinity of some transporters to sulfur-containing compounds. The obtained results considerably extend earlier large-scale studies of Rho-dependent and classic transcription attenuations.

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Keywords
Mycobacteria; Sulfur metabolism; Sulfur-containing compounds transport; Cysteine; Rho-dependent transcription attenuation; Classic transcription attenuation
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Gene expression regulation of the PF00480 or PF14340 domain proteins suggests their involvement in sulfur metabolism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 49, April 2014, Pages 7–13
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us