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Mode of action classification of chemicals using multi-concentration time-dependent cellular response profiles

Paper ID Volume ID Publish Year Pages File Format Full-Text
15073 1371 2014 13 PDF Available
Title
Mode of action classification of chemicals using multi-concentration time-dependent cellular response profiles
Abstract

•The time-dependent cellular response profiles of 65 chemicals are measured using the xCELLigence real-time cell analysis high-throughput system with treatment on human hepatocellular carcinoma cells.•A new classification method for chemicals using time-dependent cellular response profiles is proposed.•The classification results of chemicals are validated by examining common chemical mechanisms of actions.

In this paper, we present a new statistical pattern recognition method for classifying cytotoxic cellular responses to toxic agents. The advantage of the proposed method is to quickly assess the toxicity level of an unclassified toxic agent on human health by bringing cytotoxic cellular responses with similar patterns (mode of action, MoOA) into the same class. The proposed method is a model-based hierarchical classification approach incorporating principal component analysis (PCA) and functional data analysis (FDA). The cytotoxic cell responses are represented by multi-concentration time-dependent cellular response profiles (TCRPs) which are dynamically recorded by using the xCELLigence real-time cell analysis high-throughput (RTCA HT) system. The classification results obtained using our algorithm show satisfactory discrimination and are validated using biological facts by examining common chemical mechanisms of actions with treatment on human hepatocellular carcinoma cells (HepG2).

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Keywords
Mode of action; Time-dependent cellular response profiles; Principal component analysis; Functional data analysis; Hierarchical classification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 49, April 2014, Pages 23–35
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us