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Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance

Paper ID Volume ID Publish Year Pages File Format Full-Text
1516 85 2009 10 PDF Available
Title
Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance
Abstract

We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype.

Keywords
Tissue engineering; Surface topography; Contact guidance; Osteoprogenitor cells; Proteomics
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Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 4, May 2009, Pages 1137–1146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us