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Identification of novel splice variants and exons of human endothelial cell-specific chemotaxic regulator (ECSCR) by bioinformatics analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
15160 1383 2012 10 PDF Available
Title
Identification of novel splice variants and exons of human endothelial cell-specific chemotaxic regulator (ECSCR) by bioinformatics analysis
Abstract

Recent discovery of biological function of endothelial cell-specific chemotaxic regulator (ECSCR), previously known as endothelial cell-specific molecule 2 (ECSM2), in modulating endothelial cell migration, apoptosis, and angiogenesis, has made it an attractive molecule in vascular research. Thus, identification of splice variants of ECSCR could provide new strategies for better understanding its roles in health and disease. In this study, we performed a series of blast searches on the human EST database with known ECSCR cDNA sequence (Variant 1), and identified additional three splice variants (Variants 2–4). When examining the ECSCR gene in the human genome assemblies, we found a large unknown region between Exons 9 and 11. By PCR amplification and sequencing, we partially mapped Exon 10 within this previously unknown region of the ECSCR gene. Taken together, in addition to previously reported human ECSCR, we identified three novel full-length splice variants potentially encoding different protein isoforms. We further defined a total of twelve exons and nearly all exon–intron boundaries of the gene, of which only eight are annotated in current public databases. Our work provides new information on gene structure and alternative splicing of the human ECSCR, which may imply its functional complexity. This undoubtedly opens new opportunities for future investigation of the biological and pathological significance of these ECSCR splice variants.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We identify four human ECSCR splice variants (Variants 1–4) in this study. ► We define all twelve exons and exon–intron boundaries of human ECSCR gene. ► Exons 2 and 8 are novel and have not been previously annotated in public databases. ► Our data provides new information on ECSCR gene structure and alternative splicing.

Keywords
ECSCR (ECSM2); Alternative splicing; Data mining; EST alignment; Sequence homology; Exon–intron boundary
First Page Preview
Identification of novel splice variants and exons of human endothelial cell-specific chemotaxic regulator (ECSCR) by bioinformatics analysis
Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 41, December 2012, Pages 41–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering