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Identification of virtual signal transducers and activators of transcription response elements in the human insulin receptor gene promoter

Paper ID Volume ID Publish Year Pages File Format Full-Text
15188 1389 2011 3 PDF Available
Title
Identification of virtual signal transducers and activators of transcription response elements in the human insulin receptor gene promoter
Abstract

In this study, we look for the existence of signal transducers and activators of transcription response elements (STATREs) in the human insulin receptor (hIR) gene promoter and their possible relation with the estradiol-provoked transcriptional repression of the hIR gene and cellular insulin resistance in U-937 human promonocytic cells. Potential STATREs in the region from −1819 to −271 bp of the hIR gene promoter were identified by their homology with the consensus STATRE (5′TTCnnnGAA3′) using the SEQFIND programme developed in our laboratory. We located five virtual STATRE-like sites: [(I): −1472/−1464], [(II): −1548/−1540], [(III): −1552/−1544], [(IV): −1587/−1579] and [(V): −1678/−1670] showing a difference of only one base from this consensus. These STATREs-like sites were situated between 33 bp upstream the 5′ half-element of the estrogen response element 1 (ERE1)-like (−1430/−1418) and 102 bp upstream the 5′ half-element of the ERE2-like (−1567/−1555) complexed with AP-1-like sites. A principal complex constituted by STATREs (II–IV) the ERE2 and AP-1 sites (IV and V) was located between −1587/−1540 bp of the hIR gene promoter.In conclusion, these results represent the first identification of virtual STATREs in the hIR gene promoter. These STATREs appear to be specifically located in the surroundings of the two EREs overlapped by various AP-1 sites. These complexes could mediate crosstalk among STATs, estrogen receptor β (ERβ), and AP-1 regulating the ERβ-mediated transcriptional repression of the hIR gene and insulin resistance in U-937 cells.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► This is the first identification of virtual STAT response elements in the human IR promoter. ► It predicts this gene as a novel STAT target gene. ► The STATREs are located in the surroundings of the two EREs overlapped by AP-1 sites. ► These complexes could mediate crosstalk among STATs, ERβ, and AP-1. ► And regulate the E2-mediated transcriptional repression of the hIR gene and cellular insulin resistance.

Keywords
hIR promoter; Virtual STATREs; Insulin resistance
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Identification of virtual signal transducers and activators of transcription response elements in the human insulin receptor gene promoter
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Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 35, Issue 6, 14 December 2011, Pages 333–335
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us