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Sequence assembly

Paper ID Volume ID Publish Year Pages File Format Full-Text
15315 1403 2009 16 PDF Available
Title
Sequence assembly
Abstract

Despite the rapidly increasing number of sequenced and re-sequenced genomes, many issues regarding the computational assembly of large-scale sequencing data have remain unresolved. Computational assembly is crucial in large genome projects as well for the evolving high-throughput technologies and plays an important role in processing the information generated by these methods. Here, we provide a comprehensive overview of the current publicly available sequence assembly programs. We describe the basic principles of computational assembly along with the main concerns, such as repetitive sequences in genomic DNA, highly expressed genes and alternative transcripts in EST sequences. We summarize existing comparisons of different assemblers and provide a detailed descriptions and directions for download of assembly programs at: http://genome.ku.dk/resources/assembly/methods.html.

Keywords
Assembly methods; EST; Shotgun; Genomes; High-throughput sequencing
First Page Preview
Sequence assembly
Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 33, Issue 2, April 2009, Pages 121–136
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering