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A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC–MS spectrometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
15456 1414 2007 7 PDF Available
Title
A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC–MS spectrometry
Abstract

Isotope-coded affinity tags (ICAT) is a labeling technique that provides insights into quantitative molecular changes. In this paper, we propose a new protocol to identify and analyze ICAT labeled peak pairs in high-resolution LC–MS data. Our major contributions are: (1) we use isotope distance constraint, ICAT distance constraint, and LC-span constraint to identify ICAT labeled peak pairs and (2) we propose to trigger tandem MS/MS scanning based on the ratio estimation value of identified ICAT peak pairs instead of the peak intensity values. Compared with current approaches that choose peaks with high intensity values for tandem MS/MS scanning, the new protocol can improve the scanning efficiency and accuracy.

Keywords
Isotope labeling; Mass spectrometry data analysis; Tandem MS triggering; Quantitative proteomics
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A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC–MS spectrometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Computational Biology and Chemistry - Volume 31, Issue 3, June 2007, Pages 215–221
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us