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Attachment of horseradish peroxidase to polytetrafluorethylene (teflon) after plasma immersion ion implantation

Paper ID Volume ID Publish Year Pages File Format Full-Text
1553 86 2008 8 PDF Available
Title
Attachment of horseradish peroxidase to polytetrafluorethylene (teflon) after plasma immersion ion implantation
Abstract

The aim of this work was to investigate the potential of polytetrafluorethylene (PTFE) as a surface for biologically active protein attachment. A plasma immersion ion implantation (PIII) treatment was applied to PTFE to produce an activated surface for the functional attachment of the enzyme, horseradish peroxidase (HRP). Fourier transform infrared–attenuated total reflectance spectra show oxidation and carbonization of the surface layer as a function of ion fluence. The PIII treatment increases by threefold the amount of attached HRP and the activity of HRP on the modified surface is about seven times higher than that on an untreated PTFE surface. This result indicates that the PIII surface modification improves both the polymer’s protein binding capacity and its ability to retain the protein in a bioactive state.

Keywords
Polytetrafluorethylene; Plasma immersion ion implantation; Horseradish peroxidase; Protein attachment; Enzyme activity
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Attachment of horseradish peroxidase to polytetrafluorethylene (teflon) after plasma immersion ion implantation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 5, September 2008, Pages 1218–1225
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us