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Construction of caged polyplexes with a reversible intracellular unpacking property to improve stability and transfection

Paper ID Volume ID Publish Year Pages File Format Full-Text
1555 86 2008 9 PDF Available
Title
Construction of caged polyplexes with a reversible intracellular unpacking property to improve stability and transfection
Abstract

Cross-linking of protein macromonomers accompanies the assembly of viral particles, which provides the virus with high stability in the host. Following inspiration, caged polyplexes were fabricated via a biomimetic cross-linker. Thiolated polyethylenimine was synthesized and showed sufficient DNA condensation ability. Spherical particles with a diameter of about 150 nm were formed at an N/P ratio of 10. Shell-cross-linked polyplexes were then constructed by the oxidation of thiol groups in air. All the results indicate that the cross-linking shell via disulfide bonds could improve the stability of polyplexes in the physiological condition and showed a reversible unpacking property at the intracellular GSH concentration. By selecting the proper preparation conditions, polyplexes caged via a biomimetic cross-linker could efficiently release DNA for transfection.

Keywords
Non-viral gene delivery; Bioresponsive; Shell-cross-linking; Caged structure; Reversible unpacking
First Page Preview
Construction of caged polyplexes with a reversible intracellular unpacking property to improve stability and transfection
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 5, September 2008, Pages 1235–1243
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering