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Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook

Paper ID Volume ID Publish Year Pages File Format Full-Text
15633 42462 2014 7 PDF Available
Title
Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook
Abstract

•Methods for measuring and predicting fluxes in Escherichia coli knockouts are reviewed.•Keio collection of E. coli KOs facilitates systemic study of regulation and metabolism.•Recent advances in 13C-MFA permit highly precise and accurate flux measurements.•Knockout flux results are currently difficult to compare and generalize.•A high-resolution set of flux data for large number of Keio knockouts would be valuable.

Cellular metabolic and regulatory systems are of fundamental interest to biologists and engineers. Incomplete understanding of these complex systems remains an obstacle to progress in biotechnology and metabolic engineering. An established method for obtaining new information on network structure, regulation and dynamics is to study the cellular system following a perturbation such as a genetic knockout. The Keio collection of all viable Escherichia coli single-gene knockouts is facilitating a systematic investigation of the regulation and metabolism of E. coli. Of all omics measurements available, the metabolic flux profile (the fluxome) provides the most direct and relevant representation of the cellular phenotype. Recent advances in 13C-metabolic flux analysis are now permitting highly precise and accurate flux measurements for investigating cellular systems and guiding metabolic engineering efforts.

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Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 28, August 2014, Pages 127–133
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us