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Zooming in on biological processes with fluorescence nanoscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
15654 42465 2013 8 PDF Available
Title
Zooming in on biological processes with fluorescence nanoscopy
Abstract

Fluorescence nanoscopy enables the study of biological phenomena at nanometer scale spatial resolution. Recent biological studies using fluorescence nanoscopy have showcased the ability of these techniques to directly observe protein organization, subcellular molecular interactions, structural dynamics, electrical signaling, and diffusion of cytosolic proteins at unprecedented spatial resolution. Super-resolution imaging techniques critically rely on bright fluorescent probes such as organic dyes or fluorescent proteins. Recently, these methods have been extended to live cells and multicolor, three-dimensional imaging, thereby providing exquisite spatiotemporal resolutions of the order of 10–20 nm and 1–2 s for subcellular imaging. Further improvements in image processing algorithms, labeling techniques, correlative microscopy, and development of advanced fluorescent probes will be required to achieve true molecular-scale resolution using these techniques.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (203 K)Download as PowerPoint slideHighlights► Fluorescence nanoscopy can be used to decipher subcellular structures in biological systems. ► We review the underlying principles and biological application of STED, PALM, and STORM. ► For super-resolution imaging methods, there is a tradeoff between temporal and spatial resolution. ► Improving probe brightness and probe photostability are key factors for achieving higher resolution.

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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 24, Issue 4, August 2013, Pages 646–653
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us