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Quantitative single cell and single molecule proteomics for clinical studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
15666 42465 2013 7 PDF Available
Title
Quantitative single cell and single molecule proteomics for clinical studies
Abstract

•Microfluidic antibody capture chip platform, MAC chip, for counting proteins in single cells.•Multiplexing enables counting of many proteins in a single assay.•Label-free technology for analysing rare or difficult-to-access cells in patients.•Enables sensitive and accurate molecule counting in biological fluids.

A central aspect of cellular systems biology is the study of cell-to-cell variability driven by network control of molecular noise. Proteins are produced in stochastic bursts and, although time averaging smoothes their accumulated levels, variation in their copy number is substantial in members of environmental sensing and signalling networks. We have developed a label-free, microfluidic antibody capture chip platform called the MAC chip, to quantify precisely the copy numbers of many proteins from a single cell in a multiplexed single assay format. We intend to investigate protein noise in circulating tumour cells (CTCs) isolated from biopsies of cancer patients through the identification of biomolecular signatures, such as p53 tumour suppressor protein, which correlate with biological properties and clinical outcomes during treatment.

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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 24, Issue 4, August 2013, Pages 745–751
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us