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Investigating transcriptional states at single-cell-resolution

Paper ID Volume ID Publish Year Pages File Format Full-Text
15688 42468 2013 10 PDF Available
Title
Investigating transcriptional states at single-cell-resolution
Abstract

Gene expression analysis at single-cell-resolution is a powerful tool for uncovering individual cell differences within heterogeneous cell populations and complex tissues, which can provide invaluable insights into the extent of gene expression variability. Multi-dimensional information of gene expression at the level of the individual cell can help to identify distinct and rare molecular cell ‘states’ within populations and aid in unravelling genetic regulatory circuits. Gene expression analysis at the single-cell-level will also enhance our understanding of the molecular basis of aberrant cell states and disease development and holds great promise for the advancement of personalized medicine. We present approaches that provide large-scale views of gene expression at the level of the individual cell.

Graphical abstract.Figure optionsDownload full-size imageDownload high-quality image (142 K)Download as PowerPoint slideHighlights► We discuss the importance and challenges of single-cell transcript profiling methods. ► We present whole-genome single-cell RNA-Seq analysis. ► We describe microfluidic single-cell real-time PCR analysis. ► We present single-molecule RNA fluorescence in situ hybridization (FISH) techniques. ► We discuss how these approaches can be integrated. ► We comment on the choice of specific techniques.

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Investigating transcriptional states at single-cell-resolution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 24, Issue 1, February 2013, Pages 69–78
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us