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Subpopulation-proteomics in prokaryotic populations

Paper ID Volume ID Publish Year Pages File Format Full-Text
15689 42468 2013 9 PDF Available
Title
Subpopulation-proteomics in prokaryotic populations
Abstract

Clonal microbial cells do not behave in an identical manner and form subpopulations during cultivation. Besides varying micro-environmental conditions, cell inherent features like cell cycle dependent localization and concentration of regulatory proteins as well as epigenetic properties are well accepted mechanisms creating cell heterogeneity. Another suspected reason is molecular noise on the transcriptional and translational level. A promising tool to unravel reasons for cell heterogeneity is the combination of cell sorting and subpopulation proteomics. This review summarizes recent developments in prokaryotic single-cell analytics and provides a workflow for selection of single cells, low cell number mass spectrometry, and proteomics evaluation. This approach is useful for understanding the dependency of individual cell decisions on inherent protein profiles.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (177 K)Download as PowerPoint slideHighlights► The emerging field of single-cell protein analytics is reviewed. ► Antibody-based, fluorescence-based and mass spectrometry-based approaches are discussed. ► We present a workflow for the combination of flow cytometry and mass spectrometry. ► This allows monitoring of population heterogeneity on the proteomic level.

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Subpopulation-proteomics in prokaryotic populations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 24, Issue 1, February 2013, Pages 79–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us