Subcommunity FTIR-spectroscopy to determine physiological cell states
Estimation of growth potential in a complex community is a great challenge in biotechnical processes and environmental water quality control. Recently it has been shown that the macromolecular structure is a good indicator for the growth potential of phytoplankton cells. A functional understanding of natural phytoplankton communities requires a community analysis by means of single particles technologies. However, conventional biochemical methods are not sensitive enough to determine the macromolecular composition of a single cell or cell aggregates. This problem can be resolved by Fourier transform infrared (FTIR) spectroscopy, which delivers results similar to biochemical analysis with a much smaller sample size. The combined approach of flow cytometric analysis with subcommunity sorting and subsequent FTIR-analysis offers new perspectives for the understanding of community functioning and process optimization.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (193 K)Download as PowerPoint slideHighlights► FTIR spectroscopy for the analysis of the biochemical composition of phytoplankton. ► Cell separation by sorting for subsequent FTIR analysis. ► Measurement from subcommunities of natural phytoplankton samples. ► Physiological traits investigated by interpretation of FTIR spectra. ► Under the phytoplankton cell response to abiotic and biotic conditions.
Journal: Current Opinion in Biotechnology - Volume 24, Issue 1, February 2013, Pages 88–94