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Towards high resolution analysis of metabolic flux in cells and tissues

Paper ID Volume ID Publish Year Pages File Format Full-Text
15711 42470 2013 7 PDF Available
Title
Towards high resolution analysis of metabolic flux in cells and tissues
Abstract

•The metabolic state of a cell is an important determinant of cellular phenotype.•Current methods for global metabolic flux quantification characterize bulk behavior.•Genome-scale models offer promise for analyses involving multiple cell types.•Advances in metabolic imaging could afford spatiotemporally resolved analysis.

Metabolism extracts chemical energy from nutrients, uses this energy to form building blocks for biosynthesis, and interconverts between various small molecules that coordinate the activities of cellular pathways. The metabolic state of a cell is increasingly recognized to determine the phenotype of not only metabolically active cell types such as liver, muscle, and adipose, but also other specialized cell types such as neurons and immune cells. This review focuses on methods to quantify intracellular reaction flux as a measure of cellular metabolic activity, with emphasis on studies involving cells of mammalian tissue. Two key areas are highlighted for future development, single cell metabolomics and noninvasive imaging, which could enable spatiotemporally resolved analysis and thereby overcome issues of heterogeneity, a distinctive feature of tissue metabolism

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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 24, Issue 5, October 2013, Pages 933–939
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us