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The art and design of functional metagenomic screens

Paper ID Volume ID Publish Year Pages File Format Full-Text
15891 42475 2011 8 PDF Available
Title
The art and design of functional metagenomic screens
Abstract

This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host–vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.

► We describe the power and limitations of functional metagenomic screens. ► We emphasize design principles and compare host-vector systems. ► Examples of successful screens using E. coli host strains are highlighted. ► Microfluidic approaches for extending screening throughput are discussed.

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The art and design of functional metagenomic screens
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 22, Issue 3, June 2011, Pages 465–472
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us