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Isolation and characterization of antimicrobial food components

Paper ID Volume ID Publish Year Pages File Format Full-Text
16027 42482 2012 6 PDF Available
Title
Isolation and characterization of antimicrobial food components
Abstract

Nowadays there is an evident growing interest in natural antimicrobial compounds isolated from food matrices. According to the type of matrix, different isolation and purification steps are needed and as these active compounds belong to different chemical classes, also different chromatographic and electrophoretic methods coupled with various detectors (the most used diode array detector and mass spectrometer) have to be performed.This review covers recent steps made in the fundamental understanding of sample preparation methods as well as of analytical tools useful for the complete characterization of bioactive food compounds. The most commonly used methods for extraction of natural antimicrobial compounds are the conventional liquid–liquid or solid–liquid extraction and the modern techniques such as pressurized liquid extraction, microwave-assisted extraction, ultrasound-assisted extraction, solid-phase micro-extraction, supercritical fluid extraction, and matrix solid phase dispersion. The complete characterization of the compounds is achieved using both monodimensional chromatographic processes (LC, nano-LC, GC, and CE coupled with different type of detectors) and, recently, using comprehensive two-dimensional systems (LC × LC and GC × GC).

► Nowadays there is an evident growing interest in natural antimicrobial compounds. ► Depending on the source and bioactives complexity of the matrix that contains these compounds. ► Different isolation steps may be needed to provide an efficient purification. ► Natural antimicrobial compounds belong to diverse chemical classes. ► Different analytical tools may also be needed for active compounds characterization.

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Isolation and characterization of antimicrobial food components
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Publisher
Database: Elsevier - ScienceDirect
Journal: Current Opinion in Biotechnology - Volume 23, Issue 2, April 2012, Pages 168–173
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us